Mickaël Lelek, de l’équipe « Imaging and Modeling Unit » à l’Institut Pasteur à Paris, donnera un séminaire le Jeudi 12 Novembre 2015 à 11h dans la salle de conférences à XLIM. Son séminaire s’intitule « Super-resolution light microscopy ».
Note pour les doctorants : pensez à vous inscrire sur la liste d’appel lors du séminaire afin de faire valider votre participation auprès de l’école doctorale ED521 ! De plus amples informations sont disponibles sur le site de l’école doctorale (http://s2i.ed.univ-poitiers.fr/)
Abstract. Fluorescence microscopy methods are among the most popular tools in biology, as they allow to study the localization, dynamics, interactions and functions of molecules within living cells or in tissue. However, as all optical systems, microscopes have a resolution limited by the diffraction. As a consequence, in the visible spectrum it is impossible to distinguish two structures closer than ≈ 250nm. But many important biological structures or organelles such as viruses or cytoskeletal filaments have a dimensions below this limit. Over the last decade powerful technologies have been developed that can overcome the diffraction limit and achieve a resolution of ≈ 20nm or better. The importance of these methods was highlighted by the Nobel prize in chemistry in 2014, which was awarded to Stefan Hell, Eric Betzig and William Moerner, three pioneers of super-resolution microscopy. The main super-resolution techniques are Stimulated Emission Depletion microscopy (STED), Structured Illumination Microscopy (SIM), and PhotoActivatable Localization Microscopy (PALM) or Stochastic Reconstruction Microscopy (STORM). In this talk, I will first introduce the principles and relative advantages of these three methods, and then describe our group’s work on implementing, applying and improving PALM/STORM technology. In particular, I will present FLASH-PALM, a variation of PALM which allows non-invasive super-resolution imaging of delicate proteins and which we applied to study the early replication cycle of HIV. I will also describe ongoing work on the development of a dual objective PALM/STORM microscope which will enable optimal resolution in 3D.
Venez nombreux !
Contact du séminaire : louradour@xlim.fr / seminaires@xlim.fr